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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts isn't nonetheless offered.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.
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This guide outlines further more probable optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E here varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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